CREB-1 Mouse Polyclonal Antibody

SKU: orb10174

Description

CREB-1 Mouse Polyclonal Antibody

Research Area

DNA and RNA Research, Epigenetics and Nuclear Signaling, TLR Signaling Pathway, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CREB-1 (101-150/341aa)
• Target: CREB1
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Kidney (Rat) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Testis (Rat) Lysate at 40 ug, Lane 5: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CREB-1 (orb10174) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

Tissue/cell: Human endometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Mouse Anti-CREB-1 Polyclonal Antibody, Unconjugated (orb10174) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CREB1

UniProt

P16220