Cpn10 Antibody

• Catalog Number: orb1822414
• Category: Antibodies
• Description: Rabbit Anti-Human Cpn10 Polyclonal
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Reactivity: Bovine, Canine, Frog, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: Human Cpn10 peptide AA 91-101
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 10kDa
• Target: Cpn10
• Entrez: 3336
• UniProt ID: P61604
• NCBI: NP_002148.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.3, 0.02% sodium azide *Storage buffer changes when conjugated
• Alternative names: 10kDa Chaperonin Antibody, Chaperonin 10 Antibody,
• Note: For research use only
• Application notes: 1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis.

Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.