CPI-637

SKU: orb1300446

Description

CPI-637 is a cell-permeable benzodiazepinone compound that acts as a selective inhibitor of the CBP and EP300 bromodomains. It is utilized in epigenetic research to probe the roles of these acetyl-lysine readers in cellular processes and has been applied in both in vitro biochemical assays and in vivo model studies.

Research Area

Epigenetics & Chromatin

Key Properties

• CAS Number: 1884712-47-3
• MW: 386.45
• Purity: 99.89%
• Formula: C₂₂H₂₂N₆O
• SMILES: C[C@@H]1CC(=O)Nc2cccc(-c3ccc4n(C)nc(-c5cnn(C)c5)c4c3)c2N1
• Target: Epigenetic Reader Domain,Histone Acetyltransferase
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:3.87 mg/mL (10.01 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  CBP:0.03 μM|BRD4 BD1:11 μM|EP300:0.051 μM
• In Vitro:
  CPI-637 inhibits MYC expression in AMO-1 cells (EC50=0.60 μM).
• Cell Research:
  AMO-1 cells (DSMZ) were plated at 20,000 cells/well in 96-well plates in Iscove's Modified Dulbecco's Medium supplemented with 10% fetal calf serum. Cells were treated for 6 h with a dose titration of CPI-637 starting from a 5 μM top concentration. After 6 h, cells were lysed and processed for QuantiGene Plex expression analysis and data were collected on a MAGPIX multiplex Luminex instrument.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CBP, BRD4, CPI-637, CPI637, CPI 637, Epigenetic Reader Domain, EpigeneticReaderDomain, EP300, HistoneAcetyltransferase, Histone Acetyltransferase, HAT, HATs, Inhibitor, inhibit

Compound Identifiers

PubChem CID

121271792