COXI Antibody (Center)

SKU: orb1927786

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:25, WB - 1:2000
• Reactivity: Human
• Predicted Reactivity: Equine, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD46746
• Immunogen: This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids of human COXI. Antigen Region: 195-224 aa.
• Target: MT-CO1
• Molecular Weight: 57041 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, MT-CO1, COI, COXI, MTCO1

All lanes: Anti-COXI Antibody (Center) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling COXI at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and mitochondrion staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling COXI at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing endoplasmic reticulum staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

MT-CO1

UniProt

P00395