Cox2 Antibody

• Catalog Number: orb1260868
• Category: Antibodies
• Description: Cox2 Antibody
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 80kDa
• Target: PTGS2
• UniProt ID: P35354
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: COX-2, prostaglandin-endoperoxide synthase 2 (pros
• Note: For research use only
• Application notes: WB: 1:1000 - 1:4000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Cox2 antibody (orb1260868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody (orb1260868) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody (orb1260868) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using Cox2 antibody (orb1260868) at dilution of 1:100. Blue: DAPI for nuclear staining.