Coronin 1 Antibody

• Catalog Number: orb1247655
• Category: Antibodies
• Description: Coronin 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KRLDRLEETVQAK
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CORO1A
• UniProt ID: P31146
• NCBI: NP_009005.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPI
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing CORO1A and probed with orb1247655 (mock transfection in first lane).

orb1247655 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247655 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247655 (0.3 ug/ml) staining of Human Lymphoblastoid (RIPA buffer, (35 ug protein in RIPA buffer). Detected by chemiluminescence.