Copper Transporting ATPase 1 antibody

• Catalog Number: orb149795
• Category: Antibodies
• Description: Mouse monoclonal to Copper Transporting ATPase 1 (HRP). The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S60-4
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b
• Immunogen: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
• Concentration: 1 mg/ml
• Dilution range: WB (1:500), ICC/IF (1:100)
• Conjugation: HRP
• MW: 180kDa
• Target: ATP7A
• Entrez: 538
• UniProt ID: Q04656
• NCBI: NP_000043.3
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: ATP7A antibody, ATP 7A antibody, ATPase Cu transpo
• Note: For research use only
• Application notes: 1 µg/ml of SMC-398 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.