Connexin 43 Rabbit Polyclonal Antibody

• Catalog Number: orb156433
• Category: Antibodies
• Description: Connexin 43 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Connexin 43 (2-100/382aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: GJA1
• UniProt ID: P17302
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Connexin 43; connexin43; Connexin43v; Cx 43v; CX43
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): HUVEC cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Connexin 43 antibody (orb156433), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Rat Heart tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Mouse Stomach tissue lysates, Primary: Anti-Connexin 43 (orb156433) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Tissue/Cell: human brain glioma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (orb156433) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human cervix carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (orb156433) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.