Connexin 43 Antibody (N-term)

SKU: orb33234

Description

Purified rabbit polyclonal antibody.

Research Area

Cardiovascular Research, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Other

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB01369
• Immunogen: Synthetic Peptide
• Target: GJA1
• Molecular Weight: 43008
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"Gap junction alpha-1 protein antibody, anti-Connexin 43 antibody, anti-Cx43 antibody, anti-Gap junction 43 kDa heart protein" antibody

Anti-GJA1 Antibody (N121) at 1:2000 dilution + C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

(LEFT) Western blot analysis of anti-GJA1 Antibody (N-term) in CEM cell line lysates (35 ug/lane). GJA1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of hGJA1-N121.Connexin in mouse brain tissue lysates (35 ug/lane). GJA1 (arrow) was detected using the purified Pab.

Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

GJA1

UniProt

P17302

RefSeq (Protein):NP_000156.1

Gago-Fuentes, Raquel et al. The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes Oncotarget, 7, 73055-73067 (2016)

PubMed: 27682878