Connexin 36 Antibody (C-term)

• Catalog Number: orb1935255
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01385
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 36093 Da
• Target: This Connexin 36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-321 amino acids from the C-terminal region of human Connexin 36.
• UniProt ID: Q9UKL4
• NCBI: NP_065711.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Gap junction delta-2 protein, Connexin-36, Cx36, G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

All lanes: Anti-Connexin 36 Antibody (C-term) at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: rat brain lysate. Lane 4: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.