Cone-rod homeobox Antibody

• Catalog Number: orb1248368
• Category: Antibodies
• Description: Cone-rod homeobox Antibody
• Target: Cone-rod homeobox
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse, Porcine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DPLDYKDQSAWK
• UniProt ID: O43186
• MW: Approx 30kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in Rat Retina lysates (calculated MW of 32.3kDa according to Human NP_000545.1 and to Rat NP_068627.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Retina. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CRX, cone-rod homeobox, CORD2, CRD, LCA7, OTX3, co
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_000545.1

orb1248368 (1 ug/ml) staining of Rat Retina lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248368 (5 ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.