Complement C3 Antibody

SKU: orb1535278

Description

Complement C3 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000)
• Reactivity: Human, Monkey
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence QQRTFISPIKCRE, from the C-Terminus of protein sequence according to NP_000055.2.
• Target: Complement C3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3

Quick Database Links

Gene Symbol

Complement C3