Collagen VII antibody / COL7A1

SKU: orb639595

Description

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: LH7.2
• Immunogen: A recombinant full-length human Collagen VII protein was used as the immunogen for this Collagen VII antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2); Red=isotype control, Blue= LH7.2 antibody.

SDS-PAGE analysis of purified, BSA-free Collagen VII antibody (clone LH7.2) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q02388