Collagen Type V Antibody Fluorescein Conjugated

SKU: orb345835

Description

Collagen Type V antibody (FITC)

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:4,000 - 1:8,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:5,000 - 1:10,000
• Reactivity: Bovine, Human
• Application Notes:
  Anti-Collagen V Fluorescein Conjugated Antibody was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen V in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. 2-7 moles Fluorescein (FITC) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 50µL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Collagen Type V from human and bovine placenta
• Purity: Anti-Collagen V has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-Collagen Type V antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type V antibody, COL5A1 protein antibody, Collagen fetal membrane A polypeptide antibody, Collagen alpha-1 (V) chain

Biorbyt anti collagen V antibody (1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH6.0 at 99-100°C - 20 minutes for antigen retrieval.

Quick Database Links

UniProt

P25940

RefSeq (Protein):NP_056534.2