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Collagen Type III Antibody Fluorescein Conjugated
Catalog Number: orb345833
| Catalog Number | orb345833 |
|---|---|
| Category | Antibodies |
| Description | Collagen Type III antibody (FITC) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | FITC |
| Reactivity | Bovine, Human |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
| Immunogen | Collagen Type III from human and bovine placenta |
| UniProt ID | P02461 |
| Tested applications | ELISA, IHC, IP, WB |
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000 |
| Application notes | Anti-Collagen Type III Fluorescein Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-Collagen Type III antibody fluorescein Read more... |
| Note | For research use only |
| NCBI | NP_000081.1 |
Biorbyt anti collagen III antibody (1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH6.0 at 99-100°C - 20 minutes for antigen retrieval.
