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Collagen Type III Antibody Biotin Conjugated

Catalog Number: orb345870

DispatchUsually dispatched within 5-10 working days
$ 760.00
Catalog Numberorb345870
CategoryAntibodies
DescriptionCollagen Type III antibody (Biotin)
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationBiotin
ReactivityBovine, Human
Form/AppearanceLyophilized
Concentration1.0 mg/ml
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
ImmunogenCollagen Type III from human and bovine placenta
UniProt IDP02461
Tested applicationsELISA, IHC, IP, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000
Application notesAnti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Antibody TypePrimary Antibody
StorageStore vial at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesrabbit anti-Collagen Type III antibody biotin conj
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NoteFor research use only
NCBINP_000081.1
Collagen Type III Antibody Biotin Conjugated

Biorbyt anti collagen III antibody (1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH6.0 at 99-100°C - 20 minutes for antigen retrieval.