Collagen Type I Antibody Peroxidase Conjugated

• Catalog Number: orb345844
• Category: Antibodies
• Description: COL1A1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, IP, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Collagen Type I from human and bovine placenta.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:6,000, FC: 5µg/mL, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:3,000 - 1:6,000
• Form/Appearance: Lyophilized
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Conjugation: HRP
• UniProt ID: P02452
• NCBI: NP_000079.2
• Storage: Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Alternative names: rabbit anti-collagen type I antibody peroxidase co
• Note: For research use only
• Application notes: Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

qPCR and Western Blot of Anti-Collagen Type I. HDAC4 regulates HSC activation in a SIRT1-dependent manner. (C, D) Primary HSCs were transfected with indicated siRNAs. Expression levels of genes were examined by qPCR (C) and Western (D). (E, F) Primary HSCs were transfected with indicated siRNAs followed by treatment with EX-527. Expression levels of genes were examined by qPCR (E) and Western (F).

qPCR and Western Blot of Anti-Collagen Type I. SIRT1 antagonizes HSC activation via PPARγ deacetylation. (D, E) Primary mouse HSCs were infected with lentivirus carrying SIRT1 WT followed by treatment with a PPARγ antagonist (T0070907). Expression levels of fibrogenic genes were examined by qPCR and Western.