Collagen Type I Antibody Biotin Conjugated

• Catalog Number: orb345868
• Category: Antibodies
• Description: COL1A1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, FC, IHC, IP, Multiplex Assay, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Collagen Type I from human and bovine placenta.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:6,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:3,000 - 1:6,000
• Form/Appearance: Lyophilized
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Conjugation: Biotin
• UniProt ID: P02452
• NCBI: NP_000079.2
• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-collagen type I antibody biotin conjug
• Note: For research use only
• Application notes: Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Flow cytometry of Anti-Collagen Type I Antibody Biotin Conjugated. Expanded CD11chiCD123–CD14– cells are fibrocytes that mediate angiogenesis. (A) Using the same gating strategy as shown in Figure 1A, CD11chiCD123–CD14– cells from a representative subject sample were analyzed for cell surface phenotype. The shaded areas represent background fluorescence on the designated population as indicated by FMO controls. This is representative of more than 10 experiments.

Flow Cytometry of Anti-Collagen Type I Antibody Biotin Conjugated. IL-4 induces monocytes to differentiate into CD14– fibrocytes that are readily distinguished from CD14 + macrophages in the same culture. (B) Cell surface phenotype of IL-4–differentiated adherent cells identifies 2 subsets based on CD14 expression, which further shows differential expression of collagen and TSLPR. FMO controls on gated CD14 + vs CD14– populations are shown by shaded gray histograms. This is representative of more than 5 experiments from 5 separate healthy donors.

Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody (orb345868). Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.

Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody (orb345868) at 5 µg/ml for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.