COL8A2 Antibody (C-term)

SKU: orb1937904

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000, IHC-P-Leica - 1:500
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD53964
• Immunogen: This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2. Antigen Region: 571-599 aa.
• Target: COL8A2
• Molecular Weight: 67244 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha-2(VIII) chain, Endothelial collagen, COL8A2

All lanes: Anti-COL8A2 Antibody (C-term) at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Neuro-2a whole cell lysate. Lane 4: C2C12 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded Human testis tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

COL8A2

UniProt

P25067

RefSeq (Protein):NP_005193.1