COL6A1 Antibody (C-term)

• Catalog Number: orb1929960
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-858 amino acids from the C-terminal region of human COL6A1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P12109
• MW: 108529 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:500-1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Clone Number: RB19577
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Collagen alpha-1(VI) chain, COL6A1
• Note: For research use only
• NCBI: NP_001839.2

Flow cytometric analysis of 293 cells using COL6A1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of COL6A1 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of COL6A1 antibody (C-term) in Y79 cell line lysates (35 ug/lane). COL6A1 (arrow) was detected using the purified Pab.

Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.