COL5A3 rabbit pAb

• Catalog Number: orb764901
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen V
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen V alpha3. AA range:221-270
• MW: 170
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-COL5A3 antibody, anti-Collagen alpha-3(V) cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using COL5A3 Polyclonal Antibody.

Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody.

Immunofluorescence analysis of HeLa cells, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.