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Catalog Number | orb771425 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to COL17A1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human COL17A1. AA range:481-530 |
MW | 150 |
Tested applications | ELISA, WB |
Dilution range | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-COL17A1 BP180 BPAG2 antibody |
Note | For research use only |
Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of 3T3, hepg2 cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
ICC, IF | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
BF594 |
ICC, IF | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
BF647 |
ELISA | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
AE |