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| Catalog Number | orb1246645 |
|---|---|
| Category | Antibodies |
| Description | COG7 Antibody |
| Target | COG7 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: C-KTRPEDYRQVSK |
| UniProt ID | P83436 |
| Tested applications | ELISA, IHC, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 85kDa band observed in lysates cell lines HeLa, Jurkat and MOLT4 (calculated MW of 86.3kDa according to NP_705831.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | COG7, component of oligomeric golgi complex 7, CDG Read more... |
| Research Area | Cell Biology |
| Note | For research use only |
| NCBI | NP_705831.1 |

orb1246645 (0.03 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246645 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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