CNOT8 Antibody

• Catalog Number: orb1256018
• Category: Antibodies
• Description: CNOT8 Antibody
• Target: CNOT8
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CNOT8 (NP_004770.4).
• UniProt ID: Q9UFF9
• MW: Observed: 34kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCR4-NOT transcription complex subunit 8, CAF1-lik
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CNOT8 antibody (orb1256018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human esophagus using CNOT8 antibody (orb1256018) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using CNOT8 antibody (orb1256018) at dilution of 1:100 (40x lens).