CNOT4 Antibody

• Catalog Number: orb1935631
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22947
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:250, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 63510 Da
• Target: This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein.
• UniProt ID: O95628
• NCBI: NP_001177777.1, NP_037448.2, NP_001177779.1, NP_001177776.1, NP_001008226.1, NP_001177778.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CCR4-NOT transcription complex subunit 4, 632-, CC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CNOT4 Antibody in MCF-7, K562, 293 cell line lysates (35 ug/lane). CNOT4 (arrow) was detected using the purified Pab.

Anti-CNOT4 Antibody (N-Term) at 1:2000 dilution + Mouse cerebellum lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 64 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CNOT4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.