CNOT10 Antibody / CCR4-NOT transcription complex subunit 10

SKU: orb2635072

Description

CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

Images & Validation

• Tested Applications: ELISA, FACS, IF, WB
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CNOT10 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c
• Clone No.: PCRP-CNOT10-1D5
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CNOT10 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells using CNOT10 antibody (green, clone PCRP-CNOT10-1D5) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with CNOT10 antibody (blue, clone PCRP-CNOT10-1D5) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free CNOT10 antibody (clone PCRP-CNOT10-1D5) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CNOT10 antibody (clone PCRP-CNOT10-1D5). These results demonstrate the foremost specificity of the PCRP-CNOT10-1D5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

Q9H9A5