CMLD-2

SKU: orb2901021

Description

CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation.

Key Properties

• CAS Number: 958843-91-9
• MW: 513.58
• Purity: >98% (HPLC)
• Formula: C₃₁H₃₁NO₆
• SMILES: COc1ccc(cc1)C(CC(=O)N1CCCC1)c1c(OC)cc(OC)c2c(cc(=O)oc12)-c1ccccc1
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 50 mg/mL (97.36 mM; Ultrasonic )

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: SW1736, 8505C, BCPAP and K1 cells. Concentration: 1, 5, 10, 25, 35, 50, 75 μM. Incubation time: 24, 48, 72 hours. Result: Reduced the viability of all the four cell lines when used at 35, 50 and 75 μM concentration and at different time points. Apoptosis Analysis Cell line: H1299, A549, H1975, HCC827, MRC-9 and CCD16 cells. Concentration: 20, 30 μM. Incubation time: 24, 48 hours. Result: Marked activated the caspase-9 and -3 in lung tumor cells. Induce the cleavage of PARP in lung tumor cells. Significantly increased the annexin-V-positive staining in lung tumor cells. Cell Cycle Analysis Cell line: H1299, A549, MRC-9 and CCD16 cells. Concentration: 30 μM. Incubation time: 24, 48 hours. Result: Induced greater G1 phase cell cycle arrest in H1299 and A549 cells than in MRC-9 and CCD16 cells. Western blot analysis. Cell line: H1299, A549, H1975, HCC827, CCD16 and MRC-9 cells. Concentration: 20, 30 μM. Incubation time: 24, 48 hours. Result: Diminished protein expression of HuR, Bcl-2, Cyclin E and Bcl-XL and increased expression of p27 and BAX in lung tumor cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis