CMLD-2

SKU: orb1693684

Description

CMLD-2 is a potent, cell-permeable inhibitor of the HuR-ARE interaction (Kᵢ: 350 nM). It disrupts HuR's mRNA-stabilizing function, leading to downregulation of oncogenic targets like MAD2, which induces apoptosis, cell cycle arrest, and inhibits proliferation and migration in various cancer cell lines in vitro.

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 958843-91-9
• MW: 513.58
• Purity: 99.99% (May vary between batches)
• Formula: C₃₁H₃₁NO₆
• SMILES: COc1ccc(cc1)C(CC(=O)N1CCCC1)c1c(OC)cc(OC)c2c(cc(=O)oc12)-c1ccccc1
• Target: Apoptosis,HuR
• Solubility: DMSO:65 mg/mL (126.56 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.89 mM)

Bioactivity

• Target IC50:
  HuR-ARE:350 nM(Ki)
• In Vivo:
  CMLD-2 (0.3 μg, 1 μg, 3 μg ; i.pl.) attenuated NGF (left), and IL-6 (right) induced mechanical hypersensitivity during the acute and priming phase in male mice.
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• In Vitro:
  CMLD-2 (35 μM ; 72 h) impairs directional migration ability and significantly diminishes MAD2 mRNA levels in SW1736, 8505C, BCPAP, and K1 cells. CMLD-2 (20-30 μM ; 24-48 h) triggers caspase activation and induces apoptosis in H1299 and A549 cells. CMLD-2 (30 μM ; 24-48 h) causes G1 cell cycle arrest and disrupts mitochondrial function in H1299 and A549 cells. CMLD-2 (30 μM ; 24-48 h) decreases the expression of HuR and HuR-regulated mRNAs and proteins in H1299 cells. CMLD-2 (1-75 μM ; 24-72 h) suppresses thyroid cancer cell viability. CMLD-2 (0.1-10 μM) reduced the activity of mouse and human sensory neurons.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMLD 2, CMLD2, CMLD-2, Apoptosis

Compound Identifiers

PubChem CID

16746438