Clusterin Antibody / CLU / APO-J

SKU: orb3067074

Description

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, 伪 and 尾, are associated through disulfide bonds. The 尾 subunit (also called ApoJ伪) corresponds to residues 23-227. The 伪 subunit (also called ApoJ尾) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences 尾-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Clusterin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CLU/4740
• Immunogen: A portion of amino acids 150-300 from human CLU protein was used as the immunogen for the Clusterin antibody.
• Purification: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P10909