Clusterin Antibody / Apolipoprotein J / APO-J

• Catalog Number: orb1151469
• Category: Antibodies
• Description: Mouse monoclonal antibody to Clusterin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLU/4729
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 150-300 was used as the immunogen for the Apolipoprotein J antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Apolipoprotein J antibody is available for research use only.
• UniProt ID: P10909
• Storage: Aliquot the Apolipoprotein J antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Apolipoprotein J antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human testis tissue lysate using Apolipoprotein J antibody (clone CLU/4729). Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit).

IHC staining of FFPE human adrenal gland tissue with Apolipoprotein J antibody (clone CLU/4729). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Apolipoprotein J antibody (clone CLU/4729) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Apolipoprotein J antibody (clone CLU/4729). These results demonstrate the foremost specificity of the CLU/4729 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.