CLPP Antibody

• Catalog Number: orb1247668
• Category: Antibodies
• Description: CLPP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EAAPAAEPVPAST
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Heart (calculated MW of 30.2kDa according to NP_006003.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CLPP
• UniProt ID: Q16740
• NCBI: NP_006003.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CLPP, ClpP caseinolytic protease, ATP-dependent, p
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Human Heart (calculated MW of 30.2kDa according to NP_006003.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247668 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247668 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.