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Item 1 of 4
Item 1 of 4
CLNS1A Antibody (C-term)
Catalog Number: orb1930021
| Catalog Number | orb1930021 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19567 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Rabbit |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:8000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 26215 Da |
| Target | This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the C-terminal region of human CLNS1A. |
| UniProt ID | P54105 |
| NCBI | NP_001284.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Methylosome subunit pICln, Chloride channel, nucle Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of CLNS1A antibody (C-term) in HL60 cell line lysates (35 ug/lane). CLNS1A (arrow) was detected using the purified Pab.
All lanes: Anti-CLNS1A Antibody (C-term) at 1: 8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
