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CLN3 Antibody (Center)
Catalog Number: orb1788169
| Catalog Number | orb1788169 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Immunogen | 250-284 aa |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:25, FC: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 47623 Da |
| Target | This CLN3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 250-284 amino acids from the Central region of human CLN3. |
| UniProt ID | Q13286 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Battenin, Batten disease protein, Protein CLN3, CL Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded H. liver section using CLN3 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Flow cytometric analysis of Hela cells using CLN3 Antibody (Center) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from A431, KG-1 cell line, mouse kidney, mouse liver tissue lysate, SH-SY5Y cell line (from left to right), using CLN3 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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