CLEC4F Antibody (C-term)

SKU: orb1938298

Description

CLEC4F Antibody (C-term)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P-Leica - 1:250, WB - 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 65519 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLECSF13

All lanes: Anti-CLEC4F Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CLEC4F Antibody (C-term) at 1:500 dilution + CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded human skeletal muscle tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.

Quick Database Links

UniProt

Q8N1N0