CLEC4F Antibody (C-term)

• Catalog Number: orb1938298
• Category: Antibodies
• Description: CLEC4F Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8N1N0
• MW: 65519 Da
• Tested applications: IHC-P, WB
• Dilution range: IHC-P-Leica - 1:250, WB - 1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CLECSF13
• Research Area: Signal Transduction
• Note: For research use only

All lanes: Anti-CLEC4F Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CLEC4F Antibody (C-term) at 1:500 dilution + CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 66 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded human skeletal muscle tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.