CLEC4E Antibody

SKU: orb248117

Description

CLEC4 is a member of the C-type lectin family of proteins that plays a role in inflammatory and immune response. CLEC4E, also called Mincle (Macrophage-inducible C-type lectin), is found on the surface of activated macrophages and phagocytes. It is a receptor for both non-self (fungi and mycobacteria), and self (damaged cells) ligands. Dead cells release SAP130 (Sin3A-Associated Protein, 130kDa), a small nuclear ribonucleoprotein component. CLEC4E senses this and interacts with the ITAM-containing adaptor protein FcRγ, and via Syk and CARD9 signaling, activates the production of inflammatory cytokines such as TNFa, MIP-2 and IL-6.

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 2-5ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Provided assay concentrations are suggestions only, CLEC4E antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal portion of human CLEC4E was used as the immunogen for this antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human samples with CLEC4E antibody at 2 ug/ml.