CLEC4E Antibody

• Catalog Number: orb248117
• Category: Antibodies
• Description: CLEC4 is a member of the C-type lectin family of proteins that plays a role in inflammatory and immune response. CLEC4E, also called Mincle (Macrophage-inducible C-type lectin), is found on the surface of activated macrophages and phagocytes. It is a receptor for both non-self (fungi and mycobacteria), and self (damaged cells) ligands. Dead cells release SAP130 (Sin3A-Associated Protein, 130kDa), a small nuclear ribonucleoprotein component. CLEC4E senses this and interacts with the ITAM-containing adaptor protein FcRgamma, and via Syk and CARD9 signaling, activates the production of inflammatory cytokines such as TNFa, MIP-2 and IL-6.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal portion of human CLEC4E was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-5ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CLEC4E antibody is available for research use only.
• Entrez: 26253
• Storage: Aliquot the CLEC4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, CLEC4E antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using CLEC4E antibody (primary antibody dilution at: at 2 ug/ml)