CLEC2D/OCIL/LLT1 Antibody

• Catalog Number: orb1538212
• Category: Antibodies
• Description: CLEC2D/OCIL/LLT1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Equine, Human, Monkey
• Immunogen: Peptide with sequence C-LLRYKGPSDH, from the Internal region of the protein sequence according to NP_037401.1NP_001004419.1NP_001184246.1.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: CLEC2D / OCIL / LLT1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: CLEC2D, C-type lectin related f, CLAX, LLT-1, LLT1
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3958 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3958 was determined to be 3.75 ug/ml.
• Expiration Date: 12 months from date of receipt.

CLEC2D / OCIL / LLT1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

CLEC2D / OCIL / LLT1 antibody (0.3 µg/ml) staining of U937 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

CLEC2D / OCIL / LLT1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton.