Cleaved PARP Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704341
• Category: Antibodies
• Description: Cleaved PARP Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 2A10
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human PARP protein
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 89/116 kDa
• Target: PARP1
• UniProt ID: P09874
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ADP ribosyltransferase(NAD+; poly(ADP ribose) poly
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cleaved PARP antibody (orb704341), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human THP-1 cell lysates, Lane 3: Human HL60 cell lysates, Lane 4: Human K562 cell lysates, Primary: Anti-Cleaved PARP (orb704341) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 89/116 kDa, Observed band size: 100 kDa.

Sample: Lane 1: Jurkat cell lysate, Lane 2: A549 cell lysate, Primary: Anti-Cleaved PARP (orb704341) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 89/110 kD, Observed band size: 89 kD.