Cleaved-Caspase-1 p20 (D210) rabbit pAb

• Catalog Number: orb770550
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cleaved-Caspase-1 (D210)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range:161-210
• MW: 25
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CASP1 antibody, anti-IL1BC antibody, anti-IL1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.