Cleaved-CASP3 (Asp175) Antibody

• Catalog Number: orb1931055
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25833
• Tested applications: DOT, IHC-P
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IHC-P: 1:50~100, DB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31608 Da
• Target: This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved.
• UniProt ID: P42574
• NCBI: NP_116786.1, NP_004337.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Caspase-3, CASP-3, Apopain, Cysteine protease CPP3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cleaved-CASP3 (Asp175) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-CASP3 (Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Dot blot analysis of anti-Cleaved-CASP3 (Asp175) Antibody on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.