Clathrin heavy chain Rabbit mAb [KD Validated] Antibody

SKU: orb1727504

Description

Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins. Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB:1:1000~1:5000IHC:1:50~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Clathrin heavy chain
• Molecular Weight: 191kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CHC17; Clathrin heavy chain; Clathrin heavy chain like 1; CLH17; CLH22; CLTC; CLTCL; CLTD;

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Western blot analysis of Clathrin heavy chain expression in Hela cell lysate.

Quick Database Links

UniProt

Q00610, P53675