Cl-amidine hydrochloride

SKU: orb1309827

Description

Cl-amidine hydrochloride

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 1373232-26-8
• MW: 347.24
• Purity: 98.14% (May vary between batches)
• Formula: C₁₄H₂₀Cl₂N₄O₂
• SMILES: Cl.NC(=O)[C@H](CCCNC(=N)CCl)NC(=O)c1ccccc1
• Target: Apoptosis,PAD,MicroRNA
• Solubility: H2O:50 mg/mL (143.99 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.88 mM);DMSO:150 mg/mL (431.98 mM)

Bioactivity

• Target IC50:
  PAD4:5.9 μM
• In Vivo:
  METHODS: Cl-amidine (40 mg/kg) was administered intraperitoneally to mice 1 hour after cecal ligation and puncture (CLP) to observe the effect of Cl-amidine on protecting mice from sepsis-induced lethality. RESULTS Cl-amidine protected mice from sepsis-induced lethality, and Cl-amidine-treated CLP animals had a higher long-term survival rate.
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• In Vitro:
  METHODS: Mouse primary splenocytes were treated with 1 μg/mL LPS and 10 μM Cl-amidine. Cell culture supernatants were collected 6 hours after treatment. TNF-α and pro-inflammatory properties of plasma and cell culture supernatants were determined by ELISA. Cytokine expression. RESULTS Cl-amidine decreased the concentration of TNF-α in plasma. At the same time, Cl-amidine treatment could significantly reduce the concentrations of these pro-inflammatory cytokines (IL-1β, IL-6). METHODS: HT29 and TK6 cells were treated with Cl-amidine (0, 5, 10, 15, 25, 50 μg/mL, 24 hours) to detect whether Cl-amidine could induce apoptosis of HT29 and TK6 cells. RESULTS Cl-amidine induced apoptosis of these cells in a dose-dependent manner.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

colitis, Cl amidine hydrochloride, Cl-amidine, Clamidine hydrochloride, Cl-amidine Hydrochloride, Apoptosis, inhibit, miRNA, miRNA-16, miR-16, MicroRNA, microRNA-16, PAD4, Inhibitor, sepsis, Peptidylarginine Deiminase, Protein Arginine Deiminase

Compound Identifiers

PubChem CID

74889978