Cl-amidine hydrochloride

SKU: orb1218886

Description

Cl-amidine hydrochloride is an orally active peptidylarginine deminase (PAD) inhibitor (IC50: 5.9 μM for PAD4). Cl-amidine hydrochloride induces apoptosis in cancer cells and it also induces microRNA (miR)-16 in vitro causing cell cycle arrest. Cl-Amidine hydrochloride prevents histone 3 citrullination and neutrophil extracellular trap formation. It improves survival in a murine sepsis model.

Key Properties

• CAS Number: 1373232-26-8
• MW: 347.24
• Purity: >98% (HPLC)
• Formula: C₁₄H₂₀Cl₂N₄O₂
• SMILES: O=C(N[C@H](C(N)=O)CCCNC(CCl)=N)C1=CC=CC=C1.[H]Cl
• Target: Apoptosis
• Solubility: H2O:50 mg/mL (143.99 mM; Need ultrasonic);DMSO:19.23 mg/mL (55.38 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Animal model: C57BL/6 mice (8-12 wk old, DSS mouse model of colitis). Dosage: 75 mg/kg. Administration: IP once daily. Result: Suppressed PAD activity, protein citrullination, and PAD levels in the colon In vivo. Animal model: C57BL/6 mice (8-12 wk old, DSS mouse model of colitis). Dosage: 5, 25, 75 mg/kg. Administration: Oral gavage once daily. Result: Led to significant reductions in the histology scores.
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• In Vitro:
  Apoptosis Analysis. Cell line: TK6 lymphoblastoid cells and HT29 colon cancer cells. Concentration: 0, 5, 10, 15, 20, 25, 50 μg/mL. Incubation time: 24 h. Result: Induced apoptosis dose-dependently.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis