CKII alpha (CSNK2A1) Antibody (Center)

SKU: orb1928563

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Other, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB4025
• Immunogen: This CKII alpha (CSNK2A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human CKII alpha (CSNK2A1). Antigen Region: 240-269 aa.
• Target: CSNK2A1
• Molecular Weight: 45144 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Casein kinase II subunit alpha, CK II alpha, CSNK2A1, CK2A1

All lanes: Anti-CSNK2A1 Antibody (Y255) at 1:2000 dilution. Lane 1: C6 whole cell lysates. Lane 2: MOLT-4 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma

Quick Database Links

Gene Symbol

CSNK2A1

UniProt

P68400

RefSeq (Protein):NP_001886.1, NP_808228.1, NP_808227.1