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Catalog Number | orb544674 |
---|---|
Category | Antibodies |
Description | CK20 (ready to use) Mouse Monoclonal Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 6A12 |
Tested applications | ICC, IF, IHC-Fr, IHC-P |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | KLH conjugated synthetic peptide derived from human CK20 |
Antibody Type | Primary Antibody |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 48 kDa |
Target | KRT20 |
UniProt ID | P35900 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M PBS (pH7.4) with 1% rAlbumin and 0.02% Proclin300. |
Alternative names | Cytokeratin 20; CK 20; CK20; Cytokeratin20; K20; K Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (orb544674) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (orb544674) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.