Citrulline Antibody (Biotin)

SKU: orb396417

Description

Mouse monoclonal antibody against Citrulline conjugated to Biotin.

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC: (1:25) optimal dilutions for assays should be determined by the user.
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SMC-501 was sufficient for detection of Citrulline in 2 µg of Citrulline conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 6C2.1
• Immunogen: Synthetic L-Citrulline conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: Citrulline
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

L-Citrulline, 2-Amino-5-(carbamoylamino)pentanoic acid, Citrulline, Citrulline-modified protein, ACPA, Anti-citrullinated protein antibodies, Anti-citrullinated protein, Modified Citrulline, Citrulline-containing protein, Citrullinated protein

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Citrulline Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Tissue: Colon tissue. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.

Western Blot analysis of Citrulline-BSA Conjugate showing detection of 67 kDa Citrulline protein using Mouse Anti-Citrulline Monoclonal Antibody, Clone 6C2.1. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 μg). Lane 3: BSA (2.0 μg). Lane 4: Citrulline-BSA (0.5 μg). Lane 5: Citrulline-BSA (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Citrulline Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Quick Database Links

Gene Symbol

Citrulline