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CIRBP Antibody

SKU: orb1256933

Description

CIRBP Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1).
TargetCIRBP
Molecular WeightObserved: 18kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CIRBP, CIRP

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CIRBP Antibody

Western blot analysis of extracts of various cell lines, using CIRBP antibody (orb1256933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

CIRBP Antibody

Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).

CIRBP Antibody

Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).

CIRBP Antibody

Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (orb1256933) at dilution of 1:100 (20x lens).

CIRBP Antibody

Immunohistochemistry of paraffin-embedded mouse brain using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).

CIRBP Antibody

Immunohistochemistry of paraffin-embedded mouse kidney using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).

CIRBP Antibody

Immunofluorescence analysis of HeLa cells using CIRBP antibody (orb1256933). Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

CIRBP Antibody (orb1256933)

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Available Sizes

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100 μl
$ 1,000.00
DispatchUsually dispatched within 5-10 working days
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