CHGA Antibody

SKU: orb1247992

Description

CHGA Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Cerebellum and Kidney lysates. An additional band at 35kDa was observed in some lysates (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 1-2µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Small Intestine. Recommended concentration: 3.75µg/mL.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QGAKERAHQQKKH
• Target: CHGA
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

chromogranin A precursor, chromogranin A (parathyroid secretory protein 1) , CHGA, CGA , parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1

orb1247992 (0.1 ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247992 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247992 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247992 (2 ug/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247992 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

CHGA

UniProt

P10645

RefSeq (Protein):NP_001288619.1, NP_001266.1