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CHRNB4 Antibody

SKU: orb1538792

Description

CHRNB4 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P
Dilution rangeIHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000)
ReactivityHuman
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 40kD band in human testis lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kD according to NP_000741.1. The 40kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-DYRLTWNSSRYEGVN, from the internal region of the protein sequence according to NP_000741.1.
TargetCHRNB4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

CHRNB4, AChR-beta4, NAChR beta 4

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Protocol Information

IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CHRNB4 Antibody (orb1538792)

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Available Sizes

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50 μg
$ 690.00
DispatchUsually dispatched within 2-3 weeks
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