CHRNA5 Antibody

• Catalog Number: orb1249277
• Category: Antibodies
• Description: CHRNA5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HVDRYFTQKEET
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon (submucosal plexus). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CHRNA5
• UniProt ID: P30532
• NCBI: NP_000736.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CHRNA5, cholinergic receptor, nicotinic, alpha 5,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon (submucosal plexus). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249277 (1 ug/ml) staining of KELLY lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249277 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.