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CHRM1 Antibody
SKU: orb1258862
Featured
Description
Research Area
Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human CHRM1. |
| Target | CHRM1 |
| Molecular Weight | Observed: 50kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CHRM1, cholinergic receptor, muscarinic 1, HM1, M1, MGC30125, muscarinic acetylcholine receptor M1, M1R
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Western blot analysis of extracts of SH-SY5Y cells, using CHRM1 antibody (orb1258862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of OVCAR-3 cells using CHRM1 antibody (orb1258862) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using CHRM1 antibody (orb1258862) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using CHRM1 antibody (orb1258862) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of OVCAR-3 cells using CHRM1 antibody (orb1258862) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CHRM1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
CHRM1 Antibody (orb1258862)
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